TOP > 研究業績 > 原著論文・総説(英文)



  1. Fujiwara, S., Matsui, T. S., Ohashi, K., Mizuno, K., and Deguchi, S.
    Keratin-binding ability of the N-terminal Solo domain of Solo is critical for its function in cellular mechanotransduction.
    Genes Cells., in press (2019).
  2. 2018年

    1. Nagai, T., Mukoyama, S., Kagiwada, H., Goshima, N., and Mizuno, K.
      Cullin-3-KCTD10- mediated CEP97 ubiquitination promotes primary cilium formation.
      J. Cell Sci., 131 (24), jcs219527 (2018). DOI: 10.1242/jcs.219527.
    2. Nishimura, R., Kato K., Fujiwara, S., Ohashi, K., and Mizuno, K..
      Solo and keratin filaments regulate epithelial tubule morphology.
      Cell Struc. Funct., 43, 95-105 (2018).DOI: 10.1247/csf.18010
    3. Fujiwara, S., Matsui, T., Ohashi, K., Deguchi, S., and Mizuno, K.
      Solo, a RhoA-targeting guanine nucleotide exchange factor, is critical for hemidesmosome formation and acinar development in epithelial cells.
      PLoS One , 13(4), e0195124 (2018).
      DOI: 10.1371/journal.pone.0195124
    4. Wille, C., Eiseler, T., Langenberger, S. T., Richter, J., Mizuno, K., Radermacher, P., Huber-Lang, M., Seufferlein, T., and Paschke, S.
      PKD regulates actin polymerization, neutrophil deformability and transendothelial migration in response to fMLP and trauma.
      J. Leukocyte Biol., in press, (2018).
      DOI: 10.1002/JLB.4A0617-251RR
    5. Takahashi, K., Nagai, T., Chiba, S., Nakayama, K., and Mizuno K.
      Glucose deprivation induces primary cilium formation through mTORC1 inactivation.
      J. Cell Sci., 131, jcs208769 (2018).
    6. 2017年

      1. Geng, K., Kumar, S., Kimani, S. G., Kholodovych, V., Kasikara, C., Mizuno, K., Sandiford, O., Rameshwar, P., Kotenko, S. V., and Birge, R. B.
        Requirement of gamma-carboxyglutamic acid modification and phosphatidylserine binding for the activation of Tyro3, Axl, and Mertk receptors by growth arrest-specific 6.
        Front. Immunol., 8, 1521 (2017).
      2. Nagai, T., and Mizuno, K.
        Jasplakinolide induces primary cilium formation through cell rounding and YAP inactivation.
        PLoS One, 12, e0183030 (2017).
      3. Fujiwara, S., and Mizuno, K.
        Role of intermediate filaments in cell locomotion.
        Encyclopedia of Life Sciences (eLS)., A26365, John Wiley & Sons, Ltd.(2017).
      4. Abe, S., Nagai, T., Masukawa, M., Okumoto, K., Homma, Y., Fujiki, Y., and Mizuno, K.
        Localization of NDR2 to peroxisomes and its role in ciliogenesis.
        J. Biol. Chem., 292, 4089-4098 (2017).
      5. Takahashi, K., Okabe, H., Kanno, S., Nagai, T., and Mizuno, K.
        A pleckstrin homology-like domain is critical for F-actin binding and cofilin-phosphatase activity of Slingshot-1.
        Biochem. Biophys. Res. Commun., 482, 686-692 (2017).
      6. Ohashi, K., Fujiwara, S., and Mizuno, K.
        Roles of the cytoskeleton, cell adhesion, and Rho signaling in mechanosensing and mechanotransduction.
        J. Biochem., 161, 245-254 (2017).


      1. Fujiwara, S., Ohashi, K., Mashiko, T., Kondo, H., and Mizuno, K.
        Interplay between Solo and keratin filaments is critical for mechanical force-induced RhoA activation and stress fiber reinforcement.
        Mol. Biol. Cell, 27, 954-966 (2016).
      2. Morita, R., Kihara, M., Nakatsu, Y., Nomoto, Y., Ogawa, M., Ohashi, K., Mizuno, K., Tachikawa, T., Ishimoto, Y., Morishita, Y., and Tsuji, T.
        Coordination of cellular dynamics contributes to tooth epithelium deformations.
        PLoS One, 11, e0161336 (2016).
      3. Konotop, G., Bausch, E., Nagai, T., Turchinovich, A., Becker, N., Benner, A., Boutros, M., Mizuno, K., Krämer, A., and Raab, M. S.
        Pharmacological inhibition of centrosome clustering by slingshot-mediated cofilin activation and actin cortex destabilization.
        Cancer Res.,76, 6690-6700 (2016).


      1. Abiko, H., Fujiwara, S., Ohashi, K., Hiatari, R., Mashiko, T., Sakamoto, N., Sato, M., and Mizuno, K.
        Rho-guanine nucleotide exchange factors involved in cyclic stretch-induced reorientation of vascular endothelial cells.
        J. Cell Sci., 128, 1683-1695 (2015).
      2. Amagai, Y., Itoh, T., Fukuda, M., and Mizuno, K.
        Rabin8 suppresses autophagosome formation independently of its guanine nucleotide-exchange activity toward Rab8.
        J. Biochem., 158, 139-153 (2015).
      3. Ohashi, K.
        Roles of cofilin in development and its mechanisms of regulation.
        Dev. Growth Differ., 57, 275-290 (2015)
      4. Katsuno, H., Toriyama, M., Hosokawa, Y., Mizuno, K., Ikeda, K., Sakumura, Y., and Inagaki, N.
        Actin migration driven by directional assembly and disassembly of membrane-anchored actin filaments.
        Cell Reports, 12, 648-660 (2015).


      1. Ohashi, K., Sampei, K., Nakagawa, M., Uchiumi, N., Amanuma, T., Aiba, S., Oikawa, M., and Mizuno, K.
        Damnacanthal, an effective inhibitor of LIM-kinase, inhibits cell migration and invasion.
        Mol. Biol. Cell, 25, 828-840 (2014).
      2. Homma, Y., Kanno, S., Sasaki, K., Nishita, M., Yasui, A., Asano, T., Ohashi, K., and Mizuno, K.
        Insulin Receptor Substrate-4 Binds to Slingshot-1 and Promotes Cofilin Dephosphorylation.
        J. Biol. Chem., 289, 26302-26313(2014).
      3. Oda, T., Chiba, S., Nagai, T., and Mizuno, K.
        Binding to Cep164, but not EB1, is essential for centriolar localization of TTBK2 and its function in ciliogenesis.
        Genes Cells., 19, 927-940 (2014).
      4. Takahashi, K., Kanno, S., and Mizuno, K. Activation of cytosolic Slingshot-1 phosphatase by gelsolin-generated soluble actin filaments.
        Biochem. Biophys. Res. Commun., 454, 471-477 (2014).
      5. Nagai, T., and Mizuno, K.
        Multifaceted roles of Furry proteins in invertebrates and vertebrates.
        J. Biochem., 155, 137-146 (2014).
      6. Ohashi, K., and Mizuno, K.
        A novel pair of split Venus fragments for detecting protein-protein interactions by in vitro and in vivo bimolecular fluorescence complementation assays.
        "Methods in Molecular Biology: Exocytosis and Endocytosis II" (ed. by Ivanov, A. I.) Humana Press, New York, 1174, 247-262(2014).


      1. Mizuno, K.
        Signaling mechanisms and functional roles of cofilin phosphorylation and dephosphorylation.
        Cell. Signal., 25, 457-469 (2013).
      2. Chiba, S., Amagai, Y., Homma, Y., Fukuda, M., and Mizuno, K.
        NDR2-mediated Rabin8 phosphorylation is crucial for ciliogenesis by switching binding specificity.
        EMBO J., 32, 874-885 (2013).
      3. Hayashi, A., Hiatari, R., Tsuji, T., Ohashi, K., and Mizuno, K.
        p63RhoGEF-mediated formation of a single polarized lamellipodium is required for chemotactic migration of breast carcinoma cells.
        FEBS Lett., 587, 698-705 (2013).
      4. Higashida, C., Kiuchi, T., Akiba, Y., Mizuno, H., Maruoka, M., Narumiya, S., Mizuno, K., and Watanabe, N.
        F- and G-actin homeostasis regulates mechanosensitive actin nucleation by formins.
        Nat. Cell Biol., 15, 395-405 (2013).
      5. Borroni, E. M., Cancellieri, C., Vacchini, A., Benureau, Y., Lagane, B., Bachelerie, F., Arenzana-Seisdedos, F., Mizuno, K., Mantovani, A., Bonecchi, R., and Locati, M.
        beta-arrestin-dependent regulation of the cofilin pathway is required for the scavenging activity of the atypical chemokine receptor D6.
        Sci. Signal., 6, ra30 (2013).
      6. Saito, A., Miyajima, K., Akatsuka, J., Kondo, H., Mashiko, T., Kiuchi, T., Ohashi, K., and Mizuno, K.
        CaMKIIb-mediated LIM-kinase activation plays a crucial role in BDNF-induced neuritogenesis.
        Genes Cells, 18, 533-543 (2013).
      7. Hou, X., Katahira, T., Ohashi, K., Mizuno, K., Sugiyama, S, and Nakamura, H.
        Coactosin accelerates cell dynamism by promoting actin polymerization.
        Dev. Biol., 379, 53-63 (2013).
      8. Nagai, T., Ikeda, M., Chiba, S., Kanno, S., and Mizuno, K.
        Furry promotes acetylation of microtubules in the mitotic spindle via inhibition of SIRT2 tubulin-deacetylase.
        J. Cell Sci., 126, 4369-4380 (2013).


      1. Ohashi, K., Kiuchi, T., Shoji, K., Sampei, K., and Mizuno, K.
        Visualization of cofilin-actin and Ras-Raf interactions by bimolecular fluorescence complementation assays using a new pair of split Venus fragments.
        BioTechniques, 52, 45-50 (2012).
      2. Shoji, K., Ohashi, K., Sampei, K., Oikawa, M., and Mizuno, K.
        Cytochalasin D acts as an inhibitor of the actin-cofilin interaction.
        Biochem. Biophys. Res. Commun., 424, 52-57 (2012).
      3. Ikeda, M., Chiba, S., Ohashi, K., and Mizuno, K.
        Furry protein promotes Aurora A-mediated Polo-like kinase 1 activation.
        J. Biol. Chem., 287, 27670-27681 (2012).
      4. Sakuma, M., Shirai, Y., Yoshino, K., Kuramasu, M., Nakamura, T., Yanagita, T., Mizuno, K., Hide, I., Nakata, Y., and Saito, N.
        Novel PKCa-mediated phosphorylation site(s) on cofilin and their potential role in terminating histamine release.
        Mol. Biol. Cell, 23, 3707-3721 (2012).
      5. Nishio, M., Hamada, K., Kawahara, K., Sasaki, M., Noguchi, F., Chiba, S., Mizuno, K., Suzuki, S. O., Dong, Y., Tokuda, M., Morikawa, T., Hikasa, H., Eggenschwiler, J., Yabuta, N., Nojima, H., Nakagawa, K., Hata, Y., Nishina, H., Mimori, K., Mori, M., Sasaki, T., Mak, T. W., Nakano, T., Itami, S., and Suzuki, A.
        Cancer susceptibility and embryonic lethality in Mob1a/1b double-mutant mice.
        J. Clin. Invest., 122, 4505-4518 (2012).


      1. Itoh, G., Kanno, S., Uchida, K. S. K., Chiba, S., Sugino, S., Watanabe, K., Mizuno, K., Yasui, A., Hirota, T., and Tanaka, K.
        CAMP (C13orf8, ZNF828) is a novel regulator of kinetochore-microtubule attachment.
        EMBO J., 30,130-144 (2011).
      2. Kiuchi, T., Nagai, T., Ohashi, K., and Mizuno, K. Measurements of spatiotemporal changes in G-actin concentration reveal its effect on stimulus-induced actin assembly and lamellipodium extension.
        J. Cell Biol., 193,365-380 (2011).
      3. Spratley, S. J., Bastea, L. I., Doppler, H., Mizuno, K., and Storz, P.
        Protein kinase D regulates cofilin activity through p21-activated kinase 4.
        J. Biol. Chem., 286, 34254-34261 (2011).
      4. Ohashi, K., Fujiwara, S., Watanabe, T., Kondo, H., Kiuchi, T., Sato, M., and Mizuno, K.
        LIM-kinase has a dual role in regulating lamellipodium extension by decelerating the rate of actin retrograde flow and the rate of actin polymerization.
        J. Biol. Chem., 286, 36340-36351 (2011).
      5. Kiuchi, T., Nagai, T., Ohashi, K., Watanabe, N., and Mizuno, K.
        Live-cell imaging of G-actin dynamics using sequential FDAP.
        BioArchitecture, 1, 240-244 (2011)
      6. Freeman, S. A., Lei, V., Dang-Lawson, M., Mizuno, K., Roskelley, C. D., and Gold, M. R.
        Cofilin-mediated F-actin severing is regulated by the Rap GTPase and controls the cytoskeletal dynamics that drive lymphocyte spreading and BCR microcluster formation.
        J. Immunol., 187, 5887-5900 (2011).


      1. Tsuji, T., Ohta, Y., Kanno, Y., Hirose, K., Ohashi, K., and Mizuno, K.
        Involvement of p114-RhoGEF and Lfc in Wnt-3a- and Dishevelled-induced RhoA activation and neurite retraction in N1E-115 mouse neuroblastoma cells.
        Mol. Biol. Cell, 21, 3590-3600 (2010).
      2. Garg, P., Verma, R., Cook, L., Soofi, A., Venkatareddy, M., George, B., Mizuno, K., Gurniak, C., Witke, W., and Holzman, L. B.
        Actin depolymerizing factor cofilin-1 is necessary in maintaining mature podocyte architecture.
        J. Biol. Chem., 285, 22676-22688 (2010).
      3. Xiao, K., Sun, J., Kim, J., Rajagopal, S., Zhai, B., Villen, J., Haas, W., Kovacs, J. J., Shukla, A. K., Hara, M., Hernandez, M., Lachmann, A., Zhao, S., Lin, Y., Cheng, Y., Mizuno, K., Ma'ayan, A., Gygi, S. P., and Lefkowitz, R. J.
        Global phosphorylation analysis of beta-arrestin-mediated signaling downstream of a seven transmembrane receptor (7TMR).
        Proc. Natl. Acad. Sci. U.S.A., 107, 15299-15304 (2010).
      4. Mishima, T., Naotsuka, M., Horita, Y., Sato, M., Ohashi, K., and Mizuno, K.
        LIM-kinase is critical for the mesenchymal-to-amoeboid cell morphological transition in 3D matrices.
        Biochem. Biophys. Res. Commun., 392, 577-581 (2010).


      1. Toshima, J. Y., Nakanishi, J., Mizuno, K., Toshima, J., and Drubin, D. G.
        Requirements for recruitment of a G protein-coupled receptor to clathrin-coated pits in budding yeast.
        Mol. Biol. Cell, 20, 5039-5050 (2009).
      2. Eiseler, T., Doppler, H., Yan, I. K., Kitatani, K., Mizuno, K., and Storz, P.
        Protein kinase D1 regulates cofilin-mediated F-actin reorganization and cell motility via Slingshot.
        Nat. Cell Biol., 11, 545-556 (2009).
      3. Chiba, S., Ikeda, M., Katsunuma, K., Ohashi, K., and Mizuno, K.
        MST2- and Furry-mediated activation of NDR1 is critical for precise alignment of mitotic chromosomes.
        Curr. Biol., 19, 675-681 (2009).
      4. Takemura, M., Mishima, T., Wang, Y., Kasahara, J., Fukunaga, K., Ohashi, K., and Mizuno, K.
        Ca2+/calmodulin-dependent protein kinase IV-mediated LIM-kinase activation is critical for calcium signal-induced neurite outgrowth.
        J. Biol. Chem., 284, 28554-28562 (2009).


      1. Kurita, S., Watanabe, Y., Gunji, E., Ohashi, K., and Mizuno, K.
        Molecular dissection of the mechanisms of substrate recognition and F-actin-mediated activation of cofilin-phosphatase Slingshot-1.
        J. Biol. Chem., 283, 32542-32552 (2008).
      2. Kato, A., Kurita, S., Hayashi, A., Kaji, N., Ohashi, K., and Mizuno, K.
        Critical roles of actin-interacting protein 1 in cytokinesis and chemotactic migration of mammalian cells.
        Biochem. J., 414, 261-270 (2008).
      3. Kim, Y.B., Choi, S., Choi, M.C., Oh, M.A., Lee, S.A., Cho, M., Mizuno, K., Kim, S.H., and Lee, J.W.
        Cell Adhesion-dependent Cofilin Serine 3 Phosphorylation by the Integrin-linked Kinase・c-Src Complex.
        J. Biol. Chem., 283, 10089-10096 (2008).
      4. Horita, Y., Ohashi, K., Mukai, M., Inoue, M., and Mizuno, K.
        Suppression of the invasive capacity of rat ascites hepatoma cells by knockdown of Slingshot or LIM-kinase.
        J. Biol. Chem., 283, 6013-6021 (2008).
      5. San Martin, A., Lee, M. Y., Williams, H. C., Mizuno, K., Lassegue, B., and Griendling, K. K.
        Dual regulation of cofilin activity by LIM kinase and Slingshot-1L phosphatase controls Platelet-derived growth factor-induced migration of human aortic smooth muscle cells.
        Circ. Res., 102, 432-438 (2008).
      6. Kaji, N., Muramoto, A., and Mizuno, K.
        LIM-kinase-mediated cofilin phosphorylation during mitosis is required for precise spindle orientation.
        J. Biol. Chem., 283, 4983-4992 (2008).
      7. Chandramouli, S., Yu, C. Y., Yusoff, P., Lao, D. -H., Leong, H. F., Mizuno, K., and Guy, G. R.
        TESK1 interacts with Sprouty2 to abrogate its inihibition of Erk phosphorylation downstream of receptor tyrosine kinase signaling.
        J. Biol. Chem., 283, 1679-1691 (2008).


      1. Kligys, K., Claiborne, J.N., Debiase, P.J., Hopkinson, S.B., Wu, Y., Mizuno, K., and Jones, J.C.
        The slingshot family of phosphatases mediates Rac1 regulation of cofilin phosphorylation, laminin-332 organization and motility behavior of keratinocytes.
        J. Biol. Chem., 282, 32520-32528 (2007).
      2. Han, L., Stope, M.B., de Jesus, M.L., Oude Weernink, P.A., Urban, M., Wieland, T., Rosskopf, D., Mizuno, K., Jakobs, K.H., and Schmidt, M.
        Direct stimulation of receptor-controlled phospholipase D1 by phospho-cofilin.
        EMBO J., 26, 4189-4202 (2007).
      3. Iida, J, Ishizaki, H, Okamoto-Tanaka, M, Kawata, A, Sumita, K, Ohgake, S, Sato, Y, Yorifuji, H, Nukina, N, Ohashi, K, Mizuno, K, Tsutsumi, T, Mizoguchi, A, Miyoshi, J, Takai, Y, and Hata, Y.
        Synaptic scaffolding molecule alpha is a scaffold to mediate N-methyl-D-aspartate receptor-dependent RhoA activation in dendrites.
        Mol. Cell. Biol., 27, 4388-4405 (2007).
      4. Kurita, S., Gunji, E., Ohashi, K., and Mizuno, K.
        Actin filaments-stabilizing and -bundling activities of cofilin-phosphatase Slingshot-1.
        Genes Cells, 12, 663-676 (2007).
      5. Kiuchi, T., Ohashi, K., Kurita, S., and Mizuno, K.
        Cofilin promotes stimulus-induced lamellipodium formation by generating an abundant supply of actin monomers.
        J. Cell Biol., 177, 465-476 (2007).
      6. Toshima J., Toshima, J. Y., Duncan, M. C., Cope, J. T. V., Sun, Y., Martin, A. C., Anderson, S., Yates, III, J. R., Mizuno, K., and Drubin, D. G.
        Negative regulation of yeast Eps-like Arp2/3 complex activator, Pan1p, by the Hip1R-related protein, Sla2p, during endocytosis.
        Mol. Biol. Cell., 18, 658-668 (2007).
      7. Endo, M., Ohashi, K., and Mizuno, K.
        LIM-kinase and Slingshot are critical for neurite extension.
        J. Biol. Chem., 282, 13692-13702 (2007).


      1. Yamamoto, M., Nagata-Ohashi, K., Ohta, Y., Ohashi, K., and Mizuno, K.
        Identification of multiple actin-binding sites in cofilin-phosphatase Slingshot-1L.
        FEBS Lett., 580, 1789-94(2006).
      2. Kobayashi, M., Nishita, M., Mishima, T., Ohashi, K., and Mizuno, K.
        MAPKAPK-2- mediated LIM-kinase activation is critical for VEGF-induced actin remodeling and cell migration.
        EMBO J., 25, 713-26(2006).
      3. Nukada, Y., Okamoto, N., Konakahara, S., Tezuka, K., Ohashi, K., Mizuno, K., and Tsuji, T.
        AILIM/ICOS-mediated elongation of activated T cells is regulated by both the PI3-kinase/Akt and Rho family cascade.
        Int. Immunol., 18, 1815-1824 (2006).


      1. Nishita, M., Tomizawa, C., Yamamoto, M., Horita, Y., Ohashi, K., and Mizuno K.
        Spatial and temporal regulation of cofilin activity by LIM-kinase and Slingshot is critical for directional cell migration.
        J. Cell Biol., 171, 349-359 (2005).
      2. Fukuda, R., Hayashi, A., Utsunomiya, A., Nukada, Y., Fukui, R., Itoh, K., Tezuka, K., Ohashi, K., Mizuno, K., Sakamoto, M., Hamanoue, M., and Tsuji, T.
        Alteration of phosphatidylinositol 3-kinase cascade in multilobulated nuclear formation of adult T cell leukemia/lymphoma (ATLL).
        Proc. Natl. Acad. Sci. U.S.A., 102, 15213-15218 (2005).
      3. Wang, Y., Shibasaki, F., and Mizuno, K.
        Calcium signal-induced cofilin dephosphorylation is medicated by Slingshot via calcineurin.
        J. Biol. Chem., 280, 12683-12689 (2005).
      4. Tsumura, Y., Toshima, J., Leeksma, O.C., Ohashi, K., and Mizuno, K.
        Sprouty-4 negatively regulates cell spreading by inhibiting the kinase activity of testicular protein kinase.
        Biochem. J., 387, 627-637 (2005).
      5. Kaji, N., and Mizuno, K.
        Roles of cofilin, LIM-kinase,and Slingshot in cytokinesis. In Signal Transduction of Cell Divisionc (ed. by Miki, T.)
        Research Signpost, Kerala, India, pp. 125-140 (2005).


      1. Nagata-Ohashi, K., Ohta, Y., Goto, K., Chiba, S., Mori, R., Nishita, M., Ohashi, K., Kousaka, K., Iwamatsu, A., Niwa, R., Uemura, T., and Mizuno, K.
        A pathway of neuregulin-induced activation of cofilin-phosphatase Slingshot and cofilin in lamellipodia.
        J. Cell Biol., 165, 465-471 (2004).
      2. Nishita, M., Wang, Y., Tomizawa, C., Suzuki, A., Niwa, R., Uemura, T., and Mizuno, K.
        Phosphoinositide 3-kinase-mediated activation of cofilin phosphatase Slingshot and its role for insulin-induced membrane protrusion.
        J. Biol. Chem., 279, 7193-7198 (2004).
      3. Tomiyoshi, G., Horita, Y., Nishita, M., Ohashi, K., and Mizuno, K.
        Caspase-mediated cleavage and activation of LIM-kinase 1 and its role in apoptotic membrane blebbing.
        Genes Cells, 9, 591-600 (2004).
      4. Konakahara, S., Ohashi, K., Mizuno, K., Itoh, K., and Tsuji, T.
        CD29 integrin- and LIMK1/cofilin-mediated actin reorganization regulates the migration of hematopoietic progenitor cells underneath bone marrow stromal cells.
        Genes Cells, 9, 345-358 (2004).
      5. Kutsuna, H., Suzuki, K., Kamata, N., Kato, T, Hato, F., Mizuno, K., Kobayashi, H., Ishii, M., and Kitagawa, S.
        Actin reorganization and morphological changes in human neutrophils stimulated by TNF, GM-CSF and G-CSF: role of mitogen-activated protein kinases.
        Am. J. Physiol. Cell Physiol., 286, C55-C64 (2004).
      6. Verdijk, P., van Veelen, P. A., de Ru, A. H., Hensbergen, P. J., Mizuno, K., Koerten, H. K., Koning, F., Tensen, C. P., and Mommaas, A. M.
        Morphological changes during dendritic cell maturation correlate with cofilin activation and translocation to the cell membrane.
        Eur. J. Immunol., 34, 156-164 (2004).
      7. Okamoto, N., Nukada, Y., Tezuka, K., Ohashi, K., Kousaka, K., Mizuno, K, Tsuji, T.
        AILIM/ICOS-signaling induces T-cell migration/polarization of memory/effector T-cells.
        Int. Immunol., 16, 1515-1522 (2004).


      1. Endo, M., Ohashi, K., Sasaki, Y., Goshima, Y., Niwa, R., Uemura, T., and Mizuno, K.
        Control of growth cone motility and morphology by LIM-kinase and Slingshot via phosphorylation and dephosphorylation of cofilin.
        J. Neurosci., 23, 2527-2537 (2003).
      2. Fukazawa, Y., Saitoh, Y., Ozawa, F., Ohta, Y., Mizuno, K., and Inokuchi, K.
        Hippocampal LTP is accompanied by enhanced F-actin content within the dendritic spine that is essential for late LTP maintenance in vivo.
        Neuron, 38, 447-460 (2003).
      3. Kaji, N., Ohashi, K., Shuin, M., Niwa, R., Uemura, T., and Mizuno, K.
        Cell cycle-associated changes in Slingshot phosphatase activity and roles in cytokinesis in animal cells.
        J. Biol. Chem., 278, 33450-33455 (2003).
      4. Ohta, Y., Kousaka, K., Nagata-Ohashi, K., Ohashi, K., Muramoto, A., Shima, Y., Niwa, R., Uemura, T., and Mizuno, K.
        Differential activities, subcellular distribution, and tissue expression patterns of three members of Slingshot family phosphatases that dephosphorylate cofilin.
        Genes Cells, 8, 811-824 (2003).


      1. Nishita, M., Aizawa, H., and Mizuno, K.
        Stromal cell-derived factor-1alpha activates LIM-kinase 1 and induces cofilin phosphorylation for T cell chemotaxis.
        Mol. Cell. Biol., 22,774-783 (2002).
      2. Matsui, S., Matsumoto, S., Adachi, R., Kusui, K., Hirayama, A., Watanabe, H., Ohashi, K., Mizuno, K., Yamaguchi, T., Kasahara, T., and Suzuki, K.
        LIM-kinase 1 modulates opsonized zymosan-triggered activation of macrophage-like U937 cells: Possible involvement of phosphorylation of cofilin and reorganization of actin cytoskeleton.
        J. Biol. Chem., 277,544-549(2002).
      3. Niwa, R., Nagata-Ohashi, K., Takeichi, M., Mizuno, K., and Uemura, T.
        Control of actin reorganization by Slingshot, a family of phosphatases that dephosphorylate ADF/cofilin.
        Cell, 108,233-246(2002).
      4. Leeksma, O. C., van Achterberg, T. A., Tsumura, Y., Toshima, J., Eldering, E., Kroes, W. G., Mellink, C., Spaargaren, M., Mizuno, K., Pannekoek, H., and de Vries, C. J.
        Human sprouty 4, a new ras antagonist on 5q31, interacts with the dual specificity kinase TESK1.
        Eur. J. Biochem. 269, 2546-2556 (2002).
      5. Amano, T., Kaji, N., Ohashi, K., and Mizuno, K.
        Mitosis-specific activation of LIM-kinase and roles of cofilin phosphorylation and dephosphorylation in mitosis.
        J. Biol. Chem., 277, 22093-22102 (2002).


      1. Amano, T., Tanabe, K., Eto, T., Narumiya, S., and Mizuno, K.
        LIM-kinase 2 induces the formation of actin stress fibers, focal adhesions and membrane blebs, dependent on its activation by Rho-associated kinase-catalyzed phosphorylation of threonine-505.
        Biochem. J., 354, 149-159 (2001).
      2. Yanagita, M., Arai, H., Ishi, K., Nakano, T., Ohashi, K., Mizuno, K., Varnum, B., Fukatsu, A., Doi, T., Kita, T.
        Gas6 regulates mesangial cell proliferation through Axl in experimental glomerulonephritis.
        Am. J. Pathol., 158, 1423-1432 (2001).
      3. Toshima, J., Toshima, J. Y., Amano, T., Yang, N., Narumiya, S., and Mizuno, K.
        Cofilin phosphorylation by protein kinase TESK1 and its role in integrin-mediated actin reorganization and focal adhesion formation.
        Mol. Biol. Cell, 12, 1131-1145 (2001).
      4. Aizawa, H., Wakatsuki, S., Ishii, A., Moriyama, K., Sasaki, Y., Ohashi, K., Sekine-Aizawa, Y., Sehara-Fujisawa, A., Mizuno, K., Goshima, Y., and Yahara, I.
        Phosphorylation of cofilin by LIM-kinase is necessary for semaphorin 3A-induced growth cone collapse.
        Nature Neurosci., 4, 367-373 (2001).
      5. Toshima, J., Toshima, J. Y., Takeuchi, K., Mori, R., and Mizuno, K.
        Cofilin phosphorylation and actin reorganization activities of testicular protein kinase 2 and its predominant expression in testicular Sertoli cells.
        J. Biol. Chem., 276, 31449-31458 (2001).
      6. Toshima, J., Toshima, J. Y., Suzuki, M., Noda, T., and Mizuno, K.
        Cell type-specific expression of a TESK1 promoter-linked lacZ gene in transgenic mice.
        Biochem. Biophys. Res. Commun., 286, 566-573 (2001).
      7. Wakayama, T., Ohashi, K., Mizuno, K., and Iseki, S.
        Cloning and characterization of a novel mouse immunoglobulin superfamily gene expressed in early spermatogenic cells.
        Mol. Reprod. Dev., 60, 158-164 (2001).
      8. Yanagita M., Arai, H., Nakano, T., Ohashi, K., Mizuno, K., Fukatsu, A., Doi, T., and Kita, T.
        Gas6 induces mesangial cell proliferation via latent transcription factor STAT3.
        J. Biol. Chem., 276, 42364-42369 (2001).
      9. Toshima, J. Y., Toshima, J., Watanabe, T., and Mizuno, K.
        Binding of 14-3-3beta regulates the kinase activity and subcellular localization of testicular protein kinase 1.
        J. Biol. Chem., 276, 43471-43481 (2001).


      1. Ohashi, K., Nagata, K., Maekawa, M., Ishizaki, T., Narumiya, S., and Mizuno, K.
        Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine-508 within the activation loop.
        J. Biol. Chem., 275, 3577-3582 (2000).
      2. Wakayama, T., Nagata, K., Ohashi, K., Mizuno, K., Tanii, I., Yoshinaga, K., Oh-oka, T., and Toshimori, K.
        The expression and cellular localization of the sperm flagellar protein MC31/CE9 in the rat testis: possible post-transcriptional regulation during rat spermiogenesis.
        Arch. Histol. Cytol., 63, 33-41 (2000).
      3. Ishimoto, Y., Ohashi, K., Mizuno, K., and Nakano, T.
        Promotion of the uptake of phosphatidylserine liposomes and apoptotic cells by a product of growth arrest-specific gene, gas6.
        J. Biochem., 127, 411-417 (2000).
      4. Bito H., Furuyashiki, T., Ishihara, H., Shibasaki, Y., Ohashi, K., Mizuno, K., Maekawa, M., Ishizaki, T., and Narumiya, S.
        A critical role for a Rho-associated kinase, p160ROCK, in determining axon outgrowth in mammalian CNS neurons.
        Neuron, 26, 431-441 (2000).
      5. Ohashi, K., Hosoya, T., Takahashi, K., Hing, H., and Mizuno, K.
        A Drosophila LIM-kinase phosphorylates cofilin and induces actin cytoskeletal reorganization.
        Biochem. Biophys. Res. Commun., 276, 1178-1185 (2000).


      1. Toshima, J., Tanaka, T., and Mizuno, K.
        Dual specificity protein kinase activity of testis-specific protein kinase 1 and its regulation by autophosphorylation of serine-215 within the activation loop.
        J. Biol. Chem., 274, 12171-12176 (1999).
      2. Maekawa, M., Ishizaki, T., Boku, S., Watanabe, N., Fujita, A., Iwamatsu, A., Obinata, T., Ohashi, K., Mizuno, K., and Narumiya, S.
        Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase.
        Science, 285, 895-898 (1999).
      3. Yang, N., and Mizuno, K.
        Nuclear export of LIM-kinase 1, mediated by two leucine-rich nuclear export signals within the PDZ domain.
        Biochem. J., 338, 793-798 (1999).
      4. Nagata, K., Ohashi, K., Yang, N., and Mizuno, K.
        The N-terminal LIM domain negatively regulates the kinase activity of LIM-kinase 1.
        Biochem. J., 343, 99-105 (1999).
      5. Yanagita, M., Ishii, K., Ozaki, H., Arai, H., Nakano, T., Ohashi, K., Mizuno, K., Kita, T., and Doi, T.
        Mechanism of inhibitory effect of warfarin on mesangial cell proliferation.
        J. Am. Soc. Nephrol., 10, 2503-2509 (1999).
      6. Kumar, C.C., Ohashi, K., Nagata, K., Walsh, A., Bar-Sagi, D., Mizuno, K.
        SCH 51344, an inhibitor of RAS/RAC-mediated cell morphology pathway.
        Ann. N. Y. Acad. Sci., 886, 122-131 (1999).


      1. Yang, N., Higuchi, O., Ohashi, K., Nagata, K., Wada, A., Kangawa, K., Nishida, E., and Mizuno, K.
        Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization.
        Nature, 393, 809-812 (1998).
      2. Toshima, J., Nakagawara, K., Mori, M., Noda, T., and Mizuno, K.
        Structural organization and chromosomal localization of the mouse Tesk1 (testis-specific protein kinase 1) gene.
        Gene, 206, 237-245 (1998).
      3. Ikebe, C., Ohashi, K., and Mizuno, K.
        Identification of testis-specific (Limk2t) and brain-specific (Limk2c) isoforms of mouse LIM-kinase 2 gene transcripts.
        Biochem. Biophys. Res. Commun., 246, 307-312 (1998).
      4. Yang, N., Higuchi, O., and Mizuno, K.
        Cytoplasmic localization of LIM-kinase 1 is directed by a short sequence within the PDZ domain.
        Exp. Cell Res., 241, 242-252 (1998).
      5. Toshima, J., Koji, T., and Mizuno, K.
        Stage-specific expression of testis-specific protein kinase 1 (TESK1) in rat spermatogenic cells. Biochem.
        Biophys. Res. Commun., 249, 107-112 (1998)


      1. Higuchi, O., Amano, T., Yang, N., and Mizuno, K.
        Inhibition of activated Ras-induced neuronal differentiation of PC12 cells by the LIM domain of LIM-kinase 1.
        Oncogene, 14, 1819-1825 (1997).
      2. Mori, T., Okano, I., Mizuno, K., Tohyama, M., and Wanaka, A.
        Comparison of tissue distribution of two novel serine/threonine kinase genes containing the LIM motif (LIMK1 and LIMK2) in the developing rat.
        Molec. Brain Res., 45, 247-254 (1997).
      3. Tanabe, K., Nagata, K., Ohashi, K., Nakano, T., Arita, H., and Mizuno, K.
        Roles of g-carboxylation and a sex hormone-binding globulin-like domain in receptor-binding and in biological activities of Gas6.
        FEBS Lett., 408, 306-310 (1997).
      4. Nakano, T., Ishimoto, Y., Kishino, J., Umeda, M., Inoue, K., Nagata, K., Ohashi, K., Mizuno, K., and Arita, H.
        Cell adhesion to phosphatidylserine mediated by a product of growth arrest-specific gene 6.
        J. Biol. Chem., 272, 29411-29414 (1997).
      5. Ikebe, C., Ohashi, K., Fujimori, T., Bernard, O., Noda, T., Robertson, E. J., and Mizuno, K.
        Mouse LIM-kinase 2 gene: cDNA cloning, genomic organization and tissue-specificexpression of two alternatively initiated transcripts.
        Genomics, 46, 504-508 (1997).


      1. Nagata, K., Ohashi, K., Nakano, T., Arita, H., Zong, C., Hanafusa, H., and Mizuno, K.
        Identification of the product of growth arrest-specific gene 6 as a common ligand for Axl, Sky and Mer receptor tyrosine kinases.
        J. Biol. Chem., 271, 30022-30027 (1996).
      2. Kuroda, S., Tokunaga, C., Kiyohara, Y., Higuchi, O., Konishi, H., Mizuno, K., Gill, G. N., and Kikkawa, U.
        Protein-protein interaction of zinc finger LIM domains with protein kinase C.
        J. Biol. Chem., 271, 31029-31032 (1996).
      3. Higuchi, O., Baeg, G.-H., Akiyama, T., and Mizuno, K.
        Suppression of fibroblast cell growth by overexpression of LIM-kinase 1.
        FEBS Lett., 396, 81-86 (1996).
      4. Hiraoka, J., Okano, I., Higuchi, O., Neng, Y., and Mizuno, K.
        Self-association of LIM-kinase 1 mediated by interaction between an N-terminal LIM domain and a C-terminal kinase domain.
        FEBS Lett., 399, 117-121 (1996).


      1. Ohashi, K., Nagata, K., Toshima, J., Nakano, T., Arita, H., Tsuda, H., Suzuki, K., and Mizuno, K.
        Stimulation of Sky receptor tyrosine kinase by the product of growth arrest-specific gene 6.
        J. Biol. Chem., 270, 22681-22684 (1995).
      2. Okano, I., Hiraoka, J., Otera, H., Nunoue, K., Ohashi, K., Iwashita, S., Hirai, M., and Mizuno, K.
        Identification and characterization of a novel family of serine/threonine kinases containing N-terminal two LIM motifs.
        J. Biol. Chem., 270, 31321-31330 (1995).
      3. Toshima, J., Ohashi, K., Okano, I., Nunoue, K., Kishioka, M., Kuma, K., Miyata, T., Hirai, M., Baba, T., and Mizuno, K.
        Identification and characterization of a novel protein kinase, TESK1, specifically expressed in testicular germ cells.
        J. Biol. Chem., 270, 31331-31337 (1995).
      4. Nunoue, K., Ohashi, K., Okano, I, and Mizuno, K.
        LIMK-1 and LIMK-2, two members of a LIM motif-containing protein kinase family.
        Oncogene, 11, 701-710 (1995).
      5. Toshima, J., Ohashi, K., Iwashita, S., and Mizuno, K.
        Autophosphorylation activity and association with Src family kinase of Sky receptor tyrosine kinase.
        Biochem. Biophys. Res. Commun., 209, 656-663 (1995).
      6. Ohashi, K., Honda, S., Ichinomiya, N., Nakamura, T., and Mizuno, K.
        Cloning and in situ localization in the brain of rat Sky receptor tyrosine kinase.
        J. Biochem., 117, 1267-1275 (1995).

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