Title
Visualization of EXGT-mediated molecular grafting activity by means of a fluorescent-labeled xyloglucan oligomer
 
author
Ito H, Nishitani K
 
Issue
1999.--
 
Publisher
Plant Cell Physiol 40: 1172-1176
 
Abstract
A fluoresceinyl xyloglucan heptasaccharide was synthesized and used as a probe to visualize the activity of the cell wall to incorporate xyloglucan fragments into the cellulose/xyloglucan framework in suspension-cultures tobacco cells. The activity was significantly reduced in trasformant cell in which the expression of endoxyloglucan transferase (EXGT) was severely suppressed by over-ex-pression of the antisense EXGT-N1 mRNA. These results indicate the involvement of EXT in the construction of the cellulose/xyloglucan framework.
 
Pdf
PDF1
 
Comment
tobocco, BY-2 cell, XTH gene family, EXGTY-N1, molecular grafting, cell wall construction, incorporation of xyloglucan into the cell wall, fluorescent-labeled xyloglucan oligomer   http://pcp.oxfordjournals.org/cgi/content/abstract/40/11/1172?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=nishitani&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT