Visualization of EXGT-mediated molecular grafting activity by means of a fluorescent-labeled xyloglucan oligomer
Ito H, Nishitani K
Plant Cell Physiol 40: 1172-1176
A fluoresceinyl xyloglucan heptasaccharide was synthesized and used as a probe to visualize the activity of the cell wall to incorporate xyloglucan fragments into the cellulose/xyloglucan framework in suspension-cultures tobacco cells. The activity was significantly reduced in trasformant cell in which the expression of endoxyloglucan transferase (EXGT) was severely suppressed by over-ex-pression of the antisense EXGT-N1 mRNA. These results indicate the involvement of EXT in the construction of the cellulose/xyloglucan framework.