A novel method for detection of endoxyloglucan transferase
Plant Cell Physiol 33: 1159-1164
A new approach has been developed for quantification of the activity of endo-xyloglucan transferase, a novel enzyme that mediates the transfer of a segment of one xyloglucan molecule to another xyloglucan molecule. Purified xyloglucans with defined molecular-weight distributions and their fluorescent derivatives (pyridylamino xyloglucans) were used as substrates for the enzymatic reaction. The transferase activity was quantified by monitoring changes in molecular-weight distributions of substrates by an alkali compatible gel permeation chromatographic system, equipped with a pulsed amperometric detector and a fluorescence detector. This new method was applied to the rapid detection and characterization of a novel transferase derived from plant tissues.
azuki bean, XTH family of protein, VaXTH1, pyridyl amino xyloglucan, enzyme assay system for XTH (xyloglucanendotransglucosylase/hydrolses), http://pcp.oxfordjournals.org/cgi/content/abstract/33/8/1159?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=nishitani&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT