An enzyme probe for the resolution of glucuronoxylan and glucuronoarabinoxylan structures
Nishitani K, Nevins DJ
Food Hydrocolloids 5: 197-207
Recently the analysis of complex carbohydrates has been facilitated by adoption of an array of sophisticated instrumental techniques. However, The interpretation of native structures based on these methods may be compromised because (i) it is difficult to maintain structural integrity of polysaccharides during their extraction from the cell wall matrix in preparation for analysis and (ii) there are limited definitive approaches for the subsequent allocation of the mixture of extracted components into appropriate homogeneous fractions. Hence a reconciliation of biological function with specific structural entities may be obscures. As an alternative to alkali extraction we describe the use of an enzyme to dissociate fragments of glucuronoarabinoxylan from the cell wall matrix.
maize, mokecualr structural model for glucuronoxylan and glucuronoarabinoxylan