Auxin-induced changes in the cell wall xyloglucans: Effects of auxin on the two different subtractions of xyloglucans in the epicotyl cell wall of Vigna angularis
Nishitani K, Masuda Y
Plant Cell Phisiol 24: 345-355
In order to study the IAA-induced modifications of the cell wall of azuki bean (Vigna angularis Ohwi et Ohashi cv. Takara) epicotyl segments, the xyloglucans were subfractionated into two components, i.e., 4K-U and 24K xyloglucans, which were obtained by extraction with 4% KOH solution containing 8 M urea and 24% KOH solution, respectively. The weight-average molecular weights of 4K-U and 24K xyloglucans were estimated to be 40 x 104 and 106 x 104, respectively. Complete acid hydrolysis of 4K-U and 24K xyloglucans gave glucose, xylose, galactose and fucose in mole % 48.3 : 33.5 : 13.8 : 4.4 and 45.3 : 30.9 : 19.6 : 4.3, respectively. Treatment of epicotyl segments with IAA (0.1 mM) caused a decrease in the amount of 24K xyloglucans and an increase in 4K-U xyloglucans, whereas the total amount of the two xyloglucans remained constant. Furthermore, IAA treatment caused a decrease in the molecular weight of 24K xyloglucans from 106 x 104 to 78 x 104 without causing changes in their sugar compositions. With 4K-U xyloglucans, IAA caused an increase in the mole % of xylose and a decrease in the mole % of galactose and fucose.
azuki bean, epicotyl, cell wall, auxin induced cell elongation, xyloglucan fractionation, molecular weight, auxin, gel permeation chromatography  http://pcp.oxfordjournals.org/cgi/content/abstract/24/3/345?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=nishitani&searchid=1&FIRSTINDEX=10&resourcetype=HWCIT