Comprehensive approach to genes involved in cell wall modifications in Arabidopsis thaliana
Imoto K, Yokoyama R, Nishitani K
Plant Mol. Biol. 58:177–192
The plant cell wall is of supermolecular architecture, and is composed of various types of heterogeneouspolymers. A few thousand enzymes and structural proteins are directly involved in the construction processes,and in the functional aspects of the dynamic architecture in Arabidopsis thaliana. Most of theseproteins are encoded by multigene families, and most members within each family share significant similaritiesin structural features, but often exhibit differing expression profiles and physiological functions.Thus, for the molecular dissection of cell wall dynamics, it is necessary to distinguish individual memberswithin a family of proteins. As a first step towards characterizing the processes involved in cell walldynamics, we have manufactured a gene-specific 70-mer oligo microarray that consists of 765 genes classifiedinto 30 putative families of proteins that are implicated in the cell wall dynamics of Arabidopsis. Byusing this array system, we identified several sets of genes that exhibit organ preferential expression profiles.We also identified gene sets that are expressed differentially at certain specific growth stages of the Arabidopsisinflorescence stem. Our results indicate that there is a division of roles among family memberswithin each of the putative cell wall-related gene families.